Selection Markers
Trending Questions
Q.
The sequence that controls the copy number of the linked DNA in the vector, is termed?
Palindromic Sequence
Recognition Site
Selectable Marker
Ori Site
Q. An antibiotic resistance gene in a vector usually helps in the selection of:
- Competent cells
- Transformed cells
- Recombinant cells
- Multiple cloning site
Q. In genetic engineering antibiotics are used
- as selectable markers
- to select healthy vectors
- as sequences from where replication starts
- to keep the cultures free of infection.
Q. Given below are two statements: one is labelled as Assertion (A) and other is labelled as Reason (R).
Assertion (A) :
Polymerase chain reaction is used in DNA amplification
Reason (R):
The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below:
Assertion (A) :
Polymerase chain reaction is used in DNA amplification
Reason (R):
The ampicillin resistant gene is used as a selectable marker to check transformation
In the light of the above statements, choose the correct answer from the options given below:
- Both (A) and (R) are correct but (R) is not the correct explanation of (A)
- Both (A) and (R) are correct and (R) is the correct explanation of (A)
- (A) is correct but (R) is not correct
- (A) is not correct but (R) is correct
Q. The most commonly used selection marker is ampicillin resistance gene.
- True
- False
Q. Screening markers use __ gene that turns the recombinant cells white in a media that is rich is X-Gal
Q.
Hybridoma technology has been successfully used in
Production of somatic hybrids
Synthesis of haemoglobin
Production of alcohol in bulk
Synthesis of monoclonal antibodies
Q. Yeast has become important in genetic engineering because it
- Has plasmids that can be genetically engineered
- Allows the study of Eukaryotic gene relation & expression
- Grows readily & rapidly in the laboratory
- All the above
Q. Explain the importance of 'selectable marker', with the help of a suitable example. [2 Marks]
Q.
How is the coding sequence of the lac Z gene considered a better selectable marker?
Q. An antibiotic resistance gene in a vector usually helps in the selection of:
- Competent cells
- Transformed cells
- Recombinant cells
- Multiple cloning site
Q. If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______________.
- Selectable marker
- Recombinant protein
- Cloning site
- Chemical scalpels
Q. What are selectable 'markers'? What is their use in genetic engineering?
Q. Restriction sites for Pvu I and Pvu II respectively are in which genes of pBR 322
- ampR, rop
- rop, ori
- ori, rop
- rop, ampR
Q. A gene whose expression helps to identify transformed cell is known as:
- Selectable marker
- Vector
- Plasmid
- Structural gene
Q. Assertion :Recognition site should be preferably single and responsive to commonly used restriction enzymes. Reason: In pBR322 alien DNA is ligated generally in the area of Bam−HI site of tetracycline resistance gene.
- Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
- Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
- Assertion is correct but Reason is incorrect
- Both Assertion and Reason are incorrect
Q. In recombinant DNA methods, the term vector refers to:
- The enzyme that cuts DNA into restriction fragments
- The sticky end of a DNA fragment
- A plasmid used to transfer DNA into a living cell
- A DNA probe used to identify a particular gene
Q. A gene whose expression helps to identify transformed cell is known as
- Plasmid
- Selectable marker
- Vector
- Structural gene
Q. (a) Name the selectable markers in the cloning vector pBR322? Mention the role they play.
(b) Why is the coding sequence of an enzyme β-galactosidase, a preferred selectable marker in comparison to the ones named above?
(b) Why is the coding sequence of an enzyme β-galactosidase, a preferred selectable marker in comparison to the ones named above?
Q. In genetic engineering antibiotics are used
- as selectable markers
- to select healthy vectors
- as sequences from where replication starts
- to keep the cultures free of infection.
Q. Read the following statements carefully and choose the correct statements:
a. In a transcription unit, the promoter located at the 5' end of coding strand.
b. The single strand DNA having the polarity 3' → 5' is the template strand.
c. RNA polymerase binds to operator during transcription.
d. Single base DNA differences occur in humans are called Polymorphism.
a. In a transcription unit, the promoter located at the 5' end of coding strand.
b. The single strand DNA having the polarity 3' → 5' is the template strand.
c. RNA polymerase binds to operator during transcription.
d. Single base DNA differences occur in humans are called Polymorphism.
- Statements a and b
- Statements b and c
- Statements b and d
- Statements a and d
Q. (a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as "insertional inactivation"?
(b) Why is this method of selection referred to as "insertional inactivation"?
Q. Replacement of a purine N2 base with another purine base or replacement of a pyrimidine N2 base with another pyrimidine is termed as
- Transition
- Translocation
- Transcription
- Transversion
Q. Screening markers use __ gene that turns the recombinant cells white in a media that is rich is X-Gal
Q. (a) Explain how to find whether an E. coli bacterium has transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it.
(b) What does the ampicillin resistant gene act as in the above case?
(b) What does the ampicillin resistant gene act as in the above case?
Q. Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Q. Why is it essential to have selectable marker in a cloning vector?
Q. How many recombinant therapeutics are being marketed in India?
- 15
- 8
- 12
- 30
Q. What is significance of selectable marker in cloning vector?
Q.
Does plasmid contain antibiotic resistance?