Transformation

Q. Which of the following vectors can be used for cloning genes in plants and animals respectively?

1. Lamda phage and Ti plasmid
2. Lamda phage and retrovirous
3. Ti plasmid and retrovirous
4. Retrovirous and Ti plasmid

Q. In which of the following methods, gold particles are used to introduce alien DNA into host cells?

1. Micro-injection
2. Biolistics
3. Gene gun
4. Both b and c

Q. The correct sequence of steps for making a cell competent is:

I. Treatment with divalent cations
II. Heat shock ( $42°$C)
III. Placing on ice
IV. Incubation of cells with rDNA on ice

1. I → IV → II → III
2. II → IV → I → III
3. IV → II → I → III
4. I → III → IV → II

Q. A scientist used a plasmid that contained ampicillin resistance gene and lacZ gene as selectable markers. He inserted the gene of interest within the ampicillin resistance gene instead of lacZ gene due to human error. The competent cells were allowed to take up the plasmid. This was followed by plating them on agar media containing ampicillin and X-gal. Which of the following statements correctly describes the result of the experiment?

1. The bacteria which took up the recombinant plasmid would not grow
2. All of the bacteria would grow and give white colonies
3. The bacteria which took up the recombinant plasmid would form white colonies
4. The bacteria which took up the recombinant plasmid would grow and give blue colonies

Q.

Biolistic technique is used in:

1. tissue culture process

2. gene transfer process

3. hybridization process

4. germplasm conservation process

Q.

Describe the Hershey and Chase experiment. Write the conclusion drawn by the scientists after their experiment.

Q. Which of the following is not used to introduce the recombinant DNA into the host?

1. Micro-injection method
2. Gene gun method
3. Bioreactors
4. Disarmed pathogen vectors

Q. Which of the following is used to deliver genes in animal cells?

1. Ti plasmid
2. Disarmed retroviruses
3. Bacteriophage vectors
4. Both b and c

Q.

Dry apricot placed in salt solution does not swell while they swell when kept in water. Why?

Q.

In the recombinant DNA technique, the term vector refers to______

Q. Based on the figure given below which of the following statements is not correct?

1. Movement of solvent molecules will take place from chamber A to B
2. The direction and rate of osmosis depends on both the pressure gradient and concentration gradient.
   
3. Movement of solute will take place from A to B.
4. Presence of a semipermeable is a pre-requisite for this process to occur.

Q. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

1. The larger the fragment size, the farther it moves
2. Negatively charged fragments do not move
3. Positively charged fragments move to farther end
4. The smaller the fragment size, the farther it moves

Q.

RNA interference technique has been devised to protect the plants from nematode is silenced by --------- produced by the host plant

a)2000

b)20000

c)200000

d)2000000

Q. A gene is inserted within the BamH I cloning site in pBR322. Some plasmids take up the desired gene and form recombinant DNA while some fail to take it up. This mixture of plasmids is introduced into bacteria which are first grown on a medium ‘A’ containing only ampicillin. It is then replica plated on to another medium ‘B’ in which both ampicillin and tetracycline are present. Given below are the illustrations of colonies found on the two culture media. Analyse them and identify the bacterial colonies of recombinants.

1. 2, 3 and 5
2. 1 and 4
3. 1, 2, 3, 4 and 5
4. 3, 4 and 5

Q.

Is bacterial transformation a natural process?

Q.

What is the drawback of using mammalian cells as an experimental system?

Q.

Give the biological term for a solution in which the relative concentration of water molecules and solute on either side of the cell membrane is the same.

Q. Cohen and his coworkers transferred genes from S. aureus to E.coli. Which of the following statement is correct regarding this experiment?

1. An Escherichia coli plasmid was used to transfer penicillin resistance from S. aureus to E.coli
2. A Staphylococcusaureus plasmid was used to transfer tetracycline resistance gene from E. coli to S. aureus
3. E.Coli plasmid was used to transfer tetracycline resistance gene to S. aureus
4. None of the above

Q. Read the following statements and select the incorrect ones.

1. (ii) and (iii)
2. (i) and (ii)
3. (i) and (iii)
4. (iii) and (iv)

Q. Osmium tetraoxide is used in

1. Light microscopy
2. Electron microscopy
3. Phase contrast microscopy
4. X-ray diffraction

Q. What is meant by electroporation? Mention its uses.

Q. In recombinant DNA technique, the term vector refers to

1. Plasmids that can transfer foreign DNA into a living cell
2. Plasmids that can join different DNA fragments
3. Cosmids that can cut DNA at specific base sequence
4. Cosmids that can degrade harmful proteins

Q. Assertion : Reason: Reaction centre is P${}_{890}$.

1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion
3. Assertion is correct but Reason is incorrect
4. Both Assertion and Reason are incorrect

Q. Which one of the following is used as a vector for cloning of genes into higher organisms?

1. Retrovirus
2. Baculo virus
3. Rhizopus nigricans
4. Salmonella typhimurium

Q. Which of the following is not used to introduce the recombinant DNA into the host?

1. Micro-injection method
2. Gene gun method
3. Bioreactors
4. Disarmed pathogen vectors

Q. In what ways can plant tissue culture be applied for crop improvement?

Q. Cryopreservation is

1. Preservation of living beings in chemicals
2. Preservation through gases
3. Preservation at high temperature
4. Preservation at very low temperature

Q. Smoothening agent in ice-creams and brightening agent in detergents is

1. Cellulose xanthate
2. Cellulose hypoxanthate
3. Carboxymethyl cellulose
4. All the above

Q. A cell is said to be competent when it is ready to accept a foreign DNA molecule.

1. True
2. False

Q. Which of the following characteristics determines the ability to cell to form a diverse range of cells in all possible environment?

1. Differentiation
2. Cellular potency
3. Cell fate and commitment
4. All of the above