What Is DNA Fingerprinting
Trending Questions
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Annealing
Extension
Ligation
Denaturation
Satellite DNA is important because it
Codes for enzymes needed for DNA replication
Does not code for proteins and is same in all members of the population
Shows high degree of polymorphism in population and also the same degree of polymorphism in an individual, which is heritable from parents to children
Codes for proteins needed in cell cycle
What is DNA fingerprinting? Mention its application.
List The Advances In Technology That Have Improved Forensic Analysis
- double-stranded DNA oligonucleotide
- single-stranded RNA nucleotide
- single-stranded DNA nucleotide
- double-stranded DNA oligonucleotide
What is expressed sequence tags?
(i) PCR is one of the techniques used in DNA fingerprinting.
(ii) PCR can be used for detection of a specific mutation or pathogen.
(iii) Alec Jeffreys won the Nobel Prize for the development of polymerase chain reaction (PCR).
(iv) DNA primers are designed such that they can attach anywhere on the DNA segment which has to be amplified.
- i, iv
- i, ii, iii
- i, ii, iv
- iii, iv
What is DNA fingerprinting? Mention its application.
- 0.01 to 20kb
- 0.1 to 20kb
- 0.01 to 10 bp
- 0.1 to 10 bp
- 0.4 nm
- 3.4 nm
- 10 nm
- 20 nm
Which Cannot Be Used For DNA Fingerprinting In Humans?
- Autoradiography
- Isolation of DNA
- Southern blotting
- Cleavage by restriction endonucleases
- Gel electrophoresis
- I, II, IV, V, III
- II, V, III, IV, I
- II, IV, V, III, I
- V, II, III, IV, I
- DNA ligase
- Restriction endonuclease
- DNA Fragments
- E. Coli
(a) Expand VNTR and describe its role in DNA fingerprinting.
(b) List any two applications of DNA fingerprinting technique.
- Polymerase chain reaction
- Zinc finger analysis
- Restriction enzymes
- DNA - DNA hybridization
Process used for amplication or multiplication of DNA for fingerprinting is:
Nesslerisation
Northern blotting
Polymerase chain reaction
Southern blotting
Do twins have the same DNA and fingerprints?
Fill in the blanks from A to E for the steps involved in rDNA technology.
-
Isolation of DNA from plant cells primarily requires treatment of cells by __A__ enzyme.
-
DNA in gel electrophoresis moves from ___B____ to ___C___.
-
PCR is the procedure to ___D___ DNA.
-
Restriction endonuclease EcoRI was isolated from ___E___.
A: Chitinase: B: Anode; C: Cathode; D: Amplify; E: E. coli
A: Cellulase: B: Cathode; C: Anode; D: Amplify; E: E. coli
- A: Chitinase: B: Cathode; C: Anode; D: Separate; E: E. coli
A: Cellulase: B: Anode; C: Cathode; D: Isolate; E: E. coli
- In forensic science
- To amplify minisatellite
- Diagnosis of inherited disorders
- Paternity testing
- Heterogeneous nuclear RNA (hn RNA) are the smallest molecules which carry amino acids to the site of protein synthesis.
- Temin and Baltimore reported reversal of central dogma, by discovering reverse transcription in some viruses.
- In transcription only a segment of DNA and only one of the strands is copied into RNA.
- The coding DNA sequence of the gene are called exons and the intervening non-coding DNA sequences are called introns.
- Microinjection
- ELISA
- Polymerase chain reaction
- Gene gun
What are the parameters which are characteristics for DNA extraction procedures?
Match the following:
A. VNTRp. LargestB. Introns and Exonsq. DNA fingerprintingC. Dystrophinr. Bulk DNAD. Satellite DNAs. SplicingA – q, B – s, C – p, D – r
A – s, B – p, C – q, D – r
A – r, B – s, C – p, D - q
A – q, B – p, C – s, D – r
- Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
- Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
- Assertion is correct but Reason is incorrect.
- Both Assertion and Reason are incorrect.
- Central dogma
- Lac operon
- Sequence Annotation
- DNA fingerprinting
How much of human DNA is the same?
(i) Separation of DNA fragments by electrophoresis
(ii) Digestion of DNA by restriction endonucleases
(iii) Hybridization using labeled VNTR probe
(iv) Isolation of DNA
(v) Detection of hybridized DNA fragments by autoradiography
(vi) Transferring the separated DNA fragments to nitrocellulose membrane
- (ii) → (i) → (iv) → (vi) → (iii) → (v)
- (iv) → (i) → (ii) → (iii) → (vi) → (v)
- (iii) → (v) → (iv) → (ii) → (i) → (vi)
- (iv) → (ii) → (i) → (vi) → (iii) → (v)