What Is a Vector

Q.

What is a phage vector?

Q. Find the odd one out with respect to Single Cell Protein.

1. Spirulina
2. Methylophilus methylotrophus
3. Candida
4. Bacillus thuringiensis

Q.

What are cloning vectors give an example?

Q.

What is a pET vector?

Q. Assertion: Restriction endonuclease in bacteria protects them from bacteriophages.

Reason: Restriction endonucleases prevent the entry of the bacteriophage DNA.

1. Both assertion and reason are true and the reason is the correct explanation of the assertion
2. Both assertion and reason are true but the reason is not the correct explanation of the assertion
3. The assertion is true but the reason is false
4. Both the assertion and reason are false

Q. Some of the steps involved in gene cloning are given below. Arrange them in the correct order.
I. Introduction of the identified DNA into the host.
II. Selection of hosts having desired DNA.
III. Identification of DNA with desirable genes.
IV. Transfer of the desired DNA to its progeny.

1. I, II, III, IV
2. II, I, III, IV
3. III, I, II, IV
4. I, III, IV, II

Q. The Ti plasmid is chosen to be an effective plant cell vector for genetic modification because

1. it can induce tumours in all organisms
2. it is commonly available in many different plant pathogens
3. a specific segment of this plasmid integrates itself into the host genome during infection
4. it can induce tumors even after modification
   

Q. Identify the labels A, B, C, D and E and choose the correct option.

1. A - Foreign DNA, B - Sticky ends, C - EcoR I, D - Joining of DNA fragments, E - Recombinant DNA
2. A - Vector DNA, B - Foreign DNA, C - EcoR I, D - Joining of DNA fragments, E - Recombinant DNA
3. A - Gene of interest, B - Vector DNA, C - BamH I, D - DNA ligase, E - DNA fingerprinting
4. A - Plasmid , B - Gene of interest,
   C - EcoR I, D - DNA ligase, E - DNA fingerprinting

Q. Match the following columns and select the correct option.

	Column-I		Column-II
(a)	Bt cotton	(i)	Enzyme replacement therapy
(b)	Adenosine deaminase deficiency	(ii)	Silencing of specific mRNA

(c)	RNAi	(iii)	In-vitro DNA amplification
(d)	PCR	(iv)	Resistant to insect pest

1. (a) (b) (c) (d)
   (iii) (ii) (i) (iv)
2. (a) (b) (c) (d)
   (ii) (iii) (iv) (i)
3. (a) (b) (c) (d)
   (i) (ii) (iii) (iv)
4. (a) (b) (c) (d)
   (iv) (i) (ii) (iii)

Q. Read the following statements and choose the appropriate option
Statement 1 - In Griffith’s experiment, when mice were injected with heat-killed R-strain and live S-strain the mice survived.
Statement 2 - In the Hershey-Chase experiment radioisotope of phosphorus - ${}^{32}P$ was incorporated into the DNA of the viruses.
Statement 3 - In the experiment conducted by Avery, MacLeod and McCarty, they found out that in the presence of RNAse enzyme transformation of the bacteria took place.

1. Statements 1 and 2 are correct, but statement 3 is wrong
2. Statements 1 and 3 are correct, but statement 2 is wrong
3. Statements 2 and 3 are correct, but statement 1 is wrong
4. Statements 1, 2 and 3 are correct.

Q. Choose the right combination of scientists and the organisms used for their experiments
i. Griffith - Streptococcus pneumoniae, mice
ii. Griffith - Bacteriophage, mice
iii. Hershey - Chase - E.coli, $T_2$ bacteriophage
iv. Hershey - Chase - Streptococcus pneumoniae, $T_2$ bacteriophage

1. i and iii
2. ii and iii
3. i and iv
4. ii and iv

Q. Ti plasmid used in plant genetic engineering is a plasmid found naturally in

1. Clostridium butyricum
2. Rhizobium leguminosarum
3. Agrobacterium tumefaciens
4. Thermus aquaticus

Q. Which of the following is commonly used as vector for introducing a DNA fragment in human lymphocytes?

1. $\lambda$phage
2. Ti plasmid
3. Retrovirus
4. pBR 322

Q.

Does cosmids contains genes encoding viral proteins

Q. In recombinant DNA technology, the term vector refers to

1. the enzyme that cuts DNA into fragments
2. an organism capable of transmitting infectious disease pathogens
3. a plasmid used to carry DNA into a living cell
4. origin of replication

Q. In the process of recombinant DNA technology, the isolated foreign DNA is inserted in to another DNA molecule known as

1. vector
2. GMO
3. Protein vector
4. DNA finger print

Q. Which of the following is commonly used as vector for introducing a DNA fragment in human lymphocytes?

1. $\lambda$phage
2. Ti plasmid
3. pBR 322
4. Retrovirus

Q. Microbes found to be very useful in genetic engineering are

1. Escherichia coli and Agrobacterium tumefaciens
2. Vibrio cholerae and a tailed bacteriophage
3. Diplococcus sp. and Pseudomonas sp.
4. Crown gall bacterium and Caenorhabditis elegans

Q. During gene cloning, the enzyme used to join and insert DNA within plasmid is

1. DNA ligase
2. Restriction endonuclease
3. Alkaline phosphatase
4. Exonuclease

Q. First step in genetic engineering among the given options is:

1. Isolation of RNA
2. Isolation of protein
3. Purification of protein
4. Isolation of genetic material

Q. List the structural genes involved in the digestion of lactose in E coli. Highlight the function of any one. What triggers the transcription of these genes?

Q. Find the correct combination. Linkage groups.
$1$. Have genes which are linked in single chromosomes
$2$. Show independent assortment
$3$. Do not show independent assortment
$4$. In prokaryotes more than one.

1. $1,2,3$ correct
2. $2,4$ correct
3. $1,2$ correct
4. $1,3$ correct

Q. Which one of the following is a case of incorrect match?
(A) Somatic hybridisation - Fusion of two diverse cells
(B) Vector DNA - Site for tRNA synthesis
(C) Micropropagation - in vitro production of plants in large numbers
(D) Callus - Unorganised mass of cells produced in tissue culture

1. (A)
2. (B)
3. (C)
4. (D)

Q. During gene cloning, which of the following is called as a gene taxi?

1. Vaccine
2. Plasmid
3. Bacterium
4. Protozoa

Q. Which vector is commonly used in the transfer of gene in a crop plant?

1. Plasmids of B. subtilis
2. Bacteriophages
3. Ti-plasmids of Agrobacterium
4. E. coli phages

Q. Given below is a diagram of a T-4 bacteriophage. Choose the option that has all the parts correctly labelled.

1. A - Head, B - Sheath, C - Collar, D - Tail fibre
2. A - Sheath, B - Head, C - Collar, D - Tail fibre
3. A - Head, B - Collar, C - Sheath, D - Tail fibre
4. A - Collar, B - Head, C - Sheath, D - Tail fibre

Q. In specialized transduction, $\lambda$ phage transduces ............ genes of Escherichia coli.

1. trp
2. gal
3. bio
4. All the above

Q. Plasmid containing penicillinase gene is present in all strains of E. coli.

1. True
2. False

Q. Match the List-I with List-II and select the correct answer.

List-I	List-II
a. Recombinant DNA	$1$. Marker gene-reporter gene
b. Agro bacterium	$2$. DNA lipase
c. Sticky ends	$3$. T-DNA
d. Plasmids	$4$. Restriction enzymes

1. A-$3$, B-$4$, C-$1$, D-$2$
2. A-$3$, B-$4$, C-$2$, D-$1$
3. A-$4$, B-$3$, C-$1$, D-$2$
4. A-$4$, B-$3$, C-$2$, D-$1$

Q. A cell is made competent to take up foreign DNA using the following methods except ?

1. Monovalent $N{a}^{+}$
2. A microinjection.
3. Biolistics
4. Bivalent $C{a}^{++}$