What is PCR

Q. Identify the correct statement w.r.t. restriction enzyme.

1. Restriction enzymes can cut the strands of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands
2. Restriction enzymes cut the strands of DNA a little closer towards the centre of the palindrome sites, but between the two different bases on the opposite strands
3. Sticky ends are named so because they form peptide bonds with their complementary cut counterparts
4. The stickiness of the ends do not facilitate the action of DNA ligase

Q.

PCR and restriction fragment length polymorphism are methods for

1. Genetic transformation

2. Genetic fingerprinting

3. Study of enzymes

4. DNA sequencing

Q. Which of the following is a cloning vector?

1. Ori minus pBR$322$
2. Any DNA containing antibiotic resistance genes
3. Ti plasmid
4. DNA of $Salmonellatyphimurium$

Q. Glycogen is a homopolymer made of

1. Amino acids
2. Ribose units
3. Galactose units
4. Glucose units

Q. Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the

1. 5’ end
2. 3’ end
3. both 3’ and 5’ end
4. middle of 3' and 5' end

Q.

What is the composition of nucleic acid?

Q.

Explain the inheritance of Rh antigen .

Q. Eco RI restriction endonuclease requires a 6 bp sequence for recognition and cleavage. In a 10kb DNA fragment how many probable cleavage sites are present for this enzyme?

1. Zero
2. One
3. Two
4. Four
   

Q. The number of ‘ends’ in a glycogen molecule would be

1. Two, one on the left side and another on the right side
2. One
3. Equal to the number of branches plus one
4. Equal to the number of branch points

Q.

The ability of a cell to grow into a complete plant is called:

1. Cellular totipotency

2. Tissue culture

3. Somaclonal variation

4. Protoplasmic fusion

Q.

What is the significance of complementary base pairs?

Q.

How is PCR used to identify bacteria?

Q.

Satellite DNA is useful tool in

1. Genetic engineering

2. Organ transplantation

3. Sex determination

4. Forensic science

Q. Typhoid fever could be confirmed by

1. Schick test
2. Dick test
3. Mantoux test
4. Widal test

Q. In vitro clonal propagation in plants in characterized by:

1. Electrophoresis and HPLC
2. Microscopy
3. PCR and RAPD
4. Northern blotting

Q.

Explain briefly

(a) PCR

(b) Restriction enzymes and DNA

(c) Chitinase

Q. Which of the following statements is incorrect about the recognition sites of restriction endonucleases?

1. Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA
2. The recognition sequence for different endonuclease could be 4, 6, or 8 base pairs
3. The palindrome sequence reads the same on the two strands in both 5'- 3' and 3'- 5' orientations
4. Restriction endonuclease finds the restriction site by inspecting the length of the DNA

Q. The purpose of polymerase chain reaction is __________

1. DNA modification
2. DNA amplification
3. DNA replication
4. DNA visualization

Q. A certain patient is suspected to be suffering from AIDS.Which diagnostic technique will you recommend for its detection?

1. ELISA
2. MRI
3. Widal test
4. Ultrasound

Q.

Do nucleic acids contain oxygen?

Q.

What is PCR used to diagnose?

Q. T2 phage is a

1. ss DNA phage
2. ds DNA phage
3. ds RNA Phage
4. ss RNA phage

Q. Positional cloning refers to

1. Mapping a gene to a chromosomal region and then identifying and cloning a genomic copy of the gene from the region.
2. Using a selection procedure to clone a cDNA.
3. Cloning a portion of gene using PCR.
4. Isolating a gene by PCR using primers from another species.

Q. The two antiparallel strands of DNA are

1. Unequal and run in opposite directions
2. Equidistant but run in $5^{\prime }\to 3^{\prime }and{3}^{\prime }\to 5^{\prime }$ directions
3. Equidistant and run in $5^{\prime }\to 3^{\prime }$ direction
4. Unequal and diverge from each other.

Q. Construction of first recombinant DNA was done by using plasmid of

1. Escherichia coli
2. Salmonella typhimurium
3. Bacillus thuringiensis
4. Saccharomyces cerevisiae
5. Agrobacterium
   

Q.

How can an infection by a pathogen be detected?

Q. What is the main reason for PCR to be employed in rDNA technology?

1. DNA isolated from a cell sample will always be very less
2. Sometimes the DNA extraction from certain cells is very tough
3. PCR increase the chance of DNA isolation
4. PCR helps in identifying the gene of interest very easily

Q. Identify the palindromic sequence among the following.

1. 5' GAATTC 3'
   3' GAATTC 5'
2. 5' GAATTC 3'
   5' GAATTC 3'
3. 3' GAATTC 5'
   3' GAATTC 5'
4. 5' GAATTC 3'
   3' CTTAAG 5'

Q.

What happens during the first step of PCR?

Q.
[3]

a) Name the process represented by the above diagram.
b) What is the principle behind this experiment?
c) How are the bands observed?