Genetic Engineering

Q. Which of the following represents the correct sequence of steps in recombinant DNA technology?

I. Isolation of the DNA fragment or genes
II. Introduction of rDNA into the host
III. Expression of the introduced gene in the host
IV. Selection and identification of the transformed host
V. Insertion of the isolated gene in a suitable vector

1. III → II → I → V → IV
2. I → V → II → IV → III
3. V → I → III → IV → II
4. I→ III → II → IV → V

Q.

Genetic engineering is possible because:

1. We can cut DNA at specific sites by endonuclease like DNAase I.
   

2. Restriction endonuclease purified from bacteria can be used in vitro.
   

3. The phenomenon of transduction in bacteria is well understood.

4. We can see DNA by an electron microscope.

Q.

Genetic engineering has been successfully used for producing

1. transgenic mice for testing safety of polio vaccine before use in humans

2. transgenic models for studying new treatments for certain cardiac disease

3. transgenic Cow Rosie, which produces high fat milk for making ghee

4. Animals like bulls for farm work as they have super power

Q.

The first step in genetic engineering is:

1. Isolation of protein

2. Purification of protein

3. Isolation of genetic material

4. Isolation of RNA

Q. Which of the following is not a restriction endonuclease?

1. Eco RI
2. Hind III
3. Pst I
4. DNAse I

Q. Which was the first commercially grown, genetically engineered crop approved for human consumption?

1. Potato
2. Cotton
3. Maize
4. Tomato

Q. Restriction endonucleases are enzymes

1. make cuts at specific positions within the DNA molecule
2. recognise a specific nucleotide sequence for binding of DNA ligase
3. restrict the action of the enzyme DNA polymerase
4. remove nucleotides from the ends of the DNA molecule

Q. Regarding assertion and reason choose the correct option.
Assertion [A] : Insulin can be produced in bacterial cells.
Reason [R] : Genetic engineering techniques can be used to transfer genes across species.

1. [A] is true and [R] is false
2. [A] is false and [R] is true
3. Both [A] and [R] are true and [R] is a correct explanation to [A]
4. Both [A] and [R] are true but [R] is not a correct explanation to [A]

Q.

Which of the following is the correct match of enzymes with their rule in genetic engineering?

1. DNA polymerase - cutting the DNA

2. 
   Restriction enzyme - cutting the DNA

3. 
   DNA ligase - helps in DNA replication

4. 
   Restriction enzyme - helps in DNA replication.
   

Q. A kind of biotechnology involving manipulation of DNA is

1. DNA replication
2. Genetic Engineering
3. Denaturation
4. Renaturation

Q. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by .

1. gel electrophoresis
2. PCR
3. centrifugation
4. restriction mapping

Q. The altered DNA which contains DNA segments from two different organisms is called .

1. composite DNA
2. recombinant DNA
3. modified DNA
4. multi DNA

Q. Genetic engineering was developed during

1. 1970
2. 1980
3. 2000
4. 1990

Q.

Product(s) of biotechnology is/are:

1. Transgenic crops

2. Humulin

3. Bio Fertilizer

4. ​​​​​​All of the above

Q. Antibiotic resistance is passed from one bacterium to the other by the process of ______.

1. Chemotherapy
2. Cloning
3. Restriction digestion
4. Conjugation

Q.

The restriction enzyme is:

1. Endonuclease which cleaves DNA at specific sites

2. Make DNA complementary to an existing DNA or RNA

3. Cut or join DNA fragments

4. Are required in vectorless direct gene transfer