Question

Restriction endonucleases are enzymes

• A. make cuts at specific positions within the DNA molecule
• B. recognise a specific nucleotide sequence for binding of DNA ligase
• C. restrict the action of the enzyme DNA polymerase
• D. remove nucleotides from the ends of the DNA molecule

Answer 1

The correct option is A make cuts at specific positions within the DNA molecule

Restriction endonucleases recognise a specific DNA base sequence (recognition sequence, recognition site, restriction sequence or restriction site having palindromic sequence) and cleave both the strands of DNA at or near that side. The enzyme cuts the DNA, generating restriction fragments with overhanging ends or blunt ends.