PCR Cycle

Q. Which of the following steps is catalysed by Taq polymerase?

Q.

Which of the following is a correct sequence of steps in a PCR ( Polymerase Chain Reaction)?

1. Extension, Denaturation, Annealing

2. Denaturation, Annealing, Extension

3. Annealing, Denaturation, Extension

4. Denaturation, Extension, Annealing

Q.

Why is annealing important in PCR?

Q.

What is Inverse PCR?

Q.

Which of the following shows the correct sequence of steps in a polymerase chain reaction (PCR)?

1. Denaturation, annealing, and extension
2. Annealing, extension, and denaturation
3. Extension, denaturation, and annealing
4. Denaturation, extension, and annealing

Q.

What is PCR and who discovered PCR technique?

Q.

Brief about PCR.

Q.

Which is the second step in PCR?

Q.

Why is DNA polymerase used in PCR?

Q.

What are the stages of PCR?

Q.

How do you get primers for PCR?

Q. Which of the following steps is catalysed by Taq polymerase?

1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. Maintainence of high temperature during PCR

Q.

What happens in the third step of the polymerase chain reaction?

Q. Match the following steps of PCR to their respective temperatures:

1. 94$°$C
2. 72$°$C
3. 55$°$C

Q. Which of the following scientists won the Nobel prize for the development of Polymerase chain rection (PCR) in the year 1993

1. Cesar Milstein
2. Philip Sharp
3. Susumu Tonegava
4. Kary Mullis

Q.

What is a Polymerase chain reaction and why is it used?

Q. If the “annealing” step is missed during PCR, what would be its effect on the entire process?

1. DNA strands will not separate.
2. Primers will not attach.
3. DNA Polymerase would not work.
4. DNA will not extend.

Q.

What is the key to polymerase chain reaction?

Q. DNA primers are designed such that they specifically attach to the DNA fragments that we want to amplify.

1. True
2. False

Q. The extension step of PCR where DNA polymerase gets into action happens at ${72}^{\circ }C.$ This is a contrast to what we have learnt where enzymes are said to be functioning at optimum temperature range of ${35}^{\circ }to{40}^{\circ }C$. Why is it so?

1. DNA polymerase is active at low pH and high temperature
2. While ${36}^{\circ }to{40}^{\circ }C$ is the optimum temperature range, ${72}^{\circ }C$ is a special sweet spot for most enzymes to work
3. DNA polymerase used in PCR is obtained from a bacteria that thrives in hot springs
4. The temperature has no role to play in this process.

Q. DNA primers are designed such that they specifically attach to the DNA fragments that we want to amplify.

1. True
2. False

Q. The extension step of PCR where DNA polymerase gets into action happens at ${72}^{\circ }C.$ This is a contrast to what we have learnt where enzymes are said to be functioning at optimum temperature range of ${35}^{\circ }to{40}^{\circ }C$. Why is it so?

1. DNA polymerase is active at low pH and high temperature
2. While ${36}^{\circ }to{40}^{\circ }C$ is the optimum temperature range, ${72}^{\circ }C$ is a special sweet spot for most enzymes to work
3. DNA polymerase used in PCR is obtained from a bacteria that thrives in hot springs
4. The temperature has no role to play in this process.

Q. What is the most important constituent of Polymerase Chain Reaction?

1. DNA polymerase
2. DNA primers
3. DNA Sequence
4. All the above components

Q. The Taq polymerase enzyme is obtained from :

1. Thermus aquaticus
2. Thilobacillus ferroxidans
3. Bacillus subtilis
4. Pseudomonas putida