Restriction Enzyme

Q.

What bonds do restriction enzymes break?

Q.

What is BamHI restriction enzyme?

Q.

Which enzyme cleaves DNA into fragments?

Q. There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

1. coli
2. colon
3. coelom
4. coenzyme

Q.

What was the first type 2 restriction endonuclease discovered?

Q.

Which enzymes are molecular scissors?

Q. Which of the following is specifically used in genetic engineering?

1. Ligase
2. Gyrase
3. Restriction endonuclease
4. DNA polymerase

Q. A restriction endonuclease breaks bonds between the

1. base pairs of a DNA
2. base pairs of a DNA-RNA hybrid
3. sugar and phosphate components of a nucleic acid
4. exons and introns of a DNA

Q. Which of the following statements does not hold true for restriction enzyme?

1. It recognizes a palindromic nucleotide sequence
2. It is an endonuclease
3. It is isolated from viruses
4. It produces the same kind of sticky ends in different DNA molecules

Q. What process does the following diagram represent?

1. Lytic cycle
2. Lysogenic cycle
3. Action of endonucleases
4. Cloning

Q.

Is EcoRI a restriction enzyme?

Q. Which of the following pairs are correctly matched?

1. Reverse Transcriptase - DNA replication
2. DNA Ligase - Gel Electrophoresis
3. DNA Polymerase - Vector
4. Restriction enzyme – Genetic engineering

Q. Restriction enzymes present in micro-organisms do not cut their own DNA because of the presence of the group that blocks digestion.

1. methyl
2. ethyl
3. butyl
4. aromatic

Q. How many types of restriction endonucleases are present.

1. Two types
2. Three types
3. Four types
4. Five types

Q. In Cohen and Boyer's recombinant DNA experiments, restriction endonucleases were used to

1. Isolate fragments of cloned bacterial plasmids.
2. Isolate fragments of frog DNA that contained an rRNA gene.
3. Cleave the bacterial plasmid.
4. All the above

Q.

Can bacteria kill the virus?

Q. What process does the following diagram represent?

1. Lytic cycle
2. Lysogenic cycle
3. Action of endonucleases
4. Cloning

Q. The DNA sequence that the restriction enzyme recognizes and the restriction site at which it cuts are always the same.

1. False
2. True

Q.

The restriction enzyme EcoRI from E.coli is expected to cleave DNA at the following sequence

1. GAATTC

2. AAGTTC

3. AAGCTT

4. GTATATC

Q. The number of phosphodiester bonds and hydrogen bonds broken due to action of EcoRI at one recognition sequence?

1. $4\&16$
2. $2\&4$
3. $2\&8$
4. $6\&8$

Q. Restriction endonucleases are enzymes which:

1. Make cuts at specific positions within the DNA molecule
2. Recognize a specific nucleotide sequence for binding of DNA Ligase
3. Restrict the action of enzyme DNA polymerase
4. Remove nucleotides from the ends of DNA molecule