pBR322

Q. Which of the following is a plasmid?

1. pBR322
2. Bam HI
3. Eco RI
4. Hind III
5. Sal I

Q. Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

1. A - Hind I, B - EcoR I, C - ${\mathrm{amp}}^R$, D - ori
2. A - Hind I, B - BamH I, C - ${\mathrm{kan}}^R$, D - ${\mathrm{amp}}^R$
3. A - BamH I, B - Pst I, C - ori, D - ${\mathrm{amp}}^R$
4. A - EcoR I, B - BamH I, C - ${\mathrm{amp}}^R$, D - ori

Q. The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

1. amp${}^R$, tet${}^R$ - antibiotic resistance genes
2. ori - original restriction enzyme
3. rop - reduced osmotic pressure
4. Hind III, EcoRI - selectable markers

Q. Identify A, B, C and D in the given figure of E. coli cloning vector pBR322 and select the correct option.

1. A - Hind I, B - EcoR I, C - ${\mathrm{amp}}^R$, D - ori
2. A - Hind I, B - BamH I, C - ${\mathrm{kan}}^R$, D - ${\mathrm{amp}}^R$
3. A - BamH I, B - Pst I, C - ori, D - ${\mathrm{amp}}^R$
4. A - EcoR I, B - BamH I, C - ${\mathrm{amp}}^R$, D - ori

Q. The first artificial cloning vector was ________

1. pBR322
2. M13
3. Phagemid vectors
4. Cosmid vectors

Q. Which of the following(s) is/are selectable marker(s) in pBR322?

1. Methicillin resistance marker
2. Ampicillin resistance marker
3. Tetracycline resistance marker
4. Kanamycin resistance marker

Q. The ori site of pBR322 is taken from a plasmid called __.

Q. Which of the following organisms is suitable to receive pBR322?

1. Fission Yeast
2. E. coli
3. Penicillium notatum
4. S. aureus

Q. The figure below is the diagrammatic representation of the E.coli vector pBR322. Which one of the given options correctly identifies its certain components(s)?

1. Ori - original restriction enzyme
2. rop - reduced osmotic pressure
3. Hind III, Eco RI - selectable markers
4. $am{p}^R,te{t}^R$ - antibiotic resistance genes