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Question

A burglar in a huff forgot to wipe off his blood stains from the place of crime where he was involved in a theft. Name the technique which can help in identifying the burglar from the blood stains. Describe the technique.

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Solution

DNA fingerprinting is a forensic technique which identifies the individuals by their DNA characteristics. The principle is that no two individuals share exact same nucleotide sequence, just like fingerprints, hence the name DNA fingerprinting. The first step in DNA fingerprinting is the extraction of usable DNA followed by its digestion with restriction enzymes to produce the restriction fragments length polymorphisms or RFLPs which are then sorted according to size by gel electrophoresis. The sorted DNA fragments are then transferred from the gel to the surface of the nylon via southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition thereby matching different DNA samples.

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