A circular plasmid of 24.0 kB is digested with the restriction enzymes Hae III and EcoRI, then run on an agarose gel alongside a sizing ladder (right lane). Observe the results of a restriction digest of on this image of the gel electrophoresis.
What conclusion can be drawn from the data?

There is no restriction site for Eco RI, and one site for Hae III.

Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses

There is one restriction site for Eco RI and two sites for Hae III.

No worries! We‘ve got your back. Try BYJU‘S free classes today!

There is no restriction site for Eco RI and two sites for Hae III.

No worries! We‘ve got your back. Try BYJU‘S free classes today!

It is not possible to draw the above conclusions from the given data.

No worries! We‘ve got your back. Try BYJU‘S free classes today!

Open in App

Solution

The correct option is A There is no restriction site for Eco RI, and one site for Hae III.
For the circular plasmid digested by restriction enzymes Hae III and EcoRI, Hae III makes two segments of the plasmid of 16.0 and 8.0 Kbp, so Hae III has one restriction site. Whereas EcoRI the entire segment is intact that is 24.0 kbp so, it has no restriction site.
So, the correct answer is 'There is no restriction site for Eco RI, and one site for Hae III'

Suggest Corrections

Similar questions

Q. Eco RI restriction endonuclease requires a 6 bp sequence for recognition and cleavage. In a 10kb DNA fragment how many probable cleavage sites are present for this enzyme?

Q. Eco RI and Rsa I restriction endonucleases require

6

and

4

bp sequences respectively for cleavage. In a

10 k b

DNA fragment how many probable cleavage sites are present for these enzymes

Q. A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoRⅠ and one for HindⅢ. All of them are equidistant from each other. Upon incubation with both the restriction enzymes _____ band(s) appear in the gel electrophoresis.

Q. As indicated in the gel image, lanes X and Y represent samples obtained from a circular plasmid DNA after complete digestion using restriction enzyme X or Y with different recognition sites, respectively. How many sites for X and Y are present in the plasmid (sizes of the bands in kilo base pairs(kb) is shown)?

Consider the linear double stranded DND shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHI restriction enzymes. If the Product is analyzed by gel electrophoresis, how many distinct bands would be observed?

Join BYJU'S Learning Program

A circular plasmid of 24.0 kB is digested with the restriction enzymes Hae III and EcoRI, then run on an agarose gel alongside a sizing ladder (right lane). Observe the results of a restriction digest of on this image of the gel electrophoresis.What conclusion can be drawn from the data?

A circular plasmid of 24.0 kB is digested with the restriction enzymes Hae III and EcoRI, then run on an agarose gel alongside a sizing ladder (right lane). Observe the results of a restriction digest of on this image of the gel electrophoresis.
What conclusion can be drawn from the data?