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Classification of Enzymes

Consider the ...

Question

Consider the linear double stranded DND shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHI restriction enzymes. If the Product is analyzed by gel electrophoresis, how many distinct bands would be observed?

A

5

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B

2

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C

3

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D

4

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Solution

The correct option is C
3

One band = 1 kb, second band = 5 kb, third band = 3kb

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Q. Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHI restriction enzymes. If the product is analyzed by gel electrophoresis, how many distinct bands would be observed?

Q. A circular double-stranded DNA molecule of 45kb size has two restriction sites for EcoRⅠ and one for HindⅢ. All of them are equidistant from each other. Upon incubation with both the restriction enzymes _____ band(s) appear in the gel electrophoresis.

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

Q. Two DNA samples A and B, contain equal number of nucleotides and both of them have one restriction site for EcoRⅠ and Hind Ⅲ. On complete digestion with both the restriction enzymes, A forms 3 bands and B forms 2 bands on gel electrophoresis.
Based on the above statement identify the correct option.

Q. How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?

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