Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both EcoRI and BamHI restriction enzymes. If the product is analyzed by gel electrophoresis, how many distinct bands would be observed?

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Solution

The correct option is C 3
There are two sites for EcoRI and one site for BamHI. When the given DNA segment is cleaved with the two enzymes EcoRI and BamHI, the bands formed are bands of 1 kb, 5 kb and two bands of 3kb. When these DNA segments are analyzed by gel electrophoresis, there are 3 bands obtained on the basis of different sizes of the fragments.
Thus, the correct answer is option C.

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