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Question
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Solution
The correct option is A Ligase
Recombinant plasmid is a transformed plasmid formed by linking a foreign DNA (containing the gene of interest) with the plasmid DNA. This process involves the use of restriction endonucleases and ligases.
The synthesis of new DNA strands is catalyzed by polymerase III enzymes which occur in 5’→ 3’ direction, complementary to the template strand, during DNA replication.
Ligases are enzymes that catalyze the formation of phosphodiester bonds between DNA molecules resulting in the joining of two DNA molecules.
In genetic engineering it helps in joining the vector DNA with the DNA of interest to form the rDNA.
EcoR I is a restriction endonuclease obtained from Escherichia coli, that recognises the palindromic sequence 5' - GAATTC - 3' and makes a cut between G and A on both the strands to form a sticky end AATT.
Taq polymerase is an enzyme isolated from Thermus aquaticus and is highly heat resistant. It catalyzes the synthesis of polynucleotides and is used for DNA amplification through the PCR technique.
Hence the correct option is
b. Ligase
Recombinant plasmid is a transformed plasmid formed by linking a foreign DNA (containing the gene of interest) with the plasmid DNA. This process involves the use of restriction endonucleases and ligases.
The synthesis of new DNA strands is catalyzed by polymerase III enzymes which occur in 5’→ 3’ direction, complementary to the template strand, during DNA replication.
Ligases are enzymes that catalyze the formation of phosphodiester bonds between DNA molecules resulting in the joining of two DNA molecules.
In genetic engineering it helps in joining the vector DNA with the DNA of interest to form the rDNA.
EcoR I is a restriction endonuclease obtained from Escherichia coli, that recognises the palindromic sequence 5' - GAATTC - 3' and makes a cut between G and A on both the strands to form a sticky end AATT.
Taq polymerase is an enzyme isolated from Thermus aquaticus and is highly heat resistant. It catalyzes the synthesis of polynucleotides and is used for DNA amplification through the PCR technique.
Hence the correct option is
b. Ligase
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