A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
A
Eco RI
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B
taq polymerase
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C
polymerase III
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D
ligase.
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Solution
The correct option is D ligase. DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.