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Extraction of Genomic DNA

A.......... i...

Question

A.......... is a collection of DNA fragments, produced by restriction-enzymes and incorporated into plasmids.

A

DNA clone

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B

gene map

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C

DNA library

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D

DNA probe

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Solution

The correct option is C DNA library
(A) DNA clone is obtained from the technique of DNA cloning. It is a molecular biology technique used for the creation of exact replicas or clones of a particular gene or DNA using genetic engineer techniques.
(B) Gene map helps to describe the spatial arrangement of genes on chromosomes.
(C) DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study.
(D) DNA probes are used to identify and label DNA fragments that contain a specific sequence.
So the correct option is a DNA library.

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Similar questions

Q. From the below list, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
(i) Transformbacteria with recombinant DNA molecule.
(ii) Cut the plasmid DNA using restriction enzymes.
(iii) Extract plasmid DNA from bacterial cells.
(iv) Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.
(v) Use ligase to seal plasmid DNA to nonplasmid DNA.

Q. How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?

Q. A.......... is multiple, identical copies of a collection of DNA fragments inserted into plasmids

Q. How many of the following statements are correct?
I. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites, 4 fragments would be produced.
II. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 6 fragments would be produced.
III. If a linear DNA molecule is digested with a restriction enzyme at four recognition sites on the DNA, 5 fragments would be produced.
IV. If a closed circular DNA molecule is digested with a restriction enzyme at six recognition sites, 7 fragments would be produced.

Q. Arrange the following steps of formation of recombinant DNA in the correct sequence.
I. Complementary sticky ends are produced on the foreign DNA and the plasmid.
II. Foreign DNA and plasmids are mixed together
III. DNA ligase is used to seal the nicks in the sugar–phosphate backbone.
IV. Cut the foreign DNA (containing the DNA fragment of interest) and the plasmid with the same restriction enzyme.

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