Question

(a) List the two methodologies which were involved in human genome project. Mention how they were used.

(b) Expand "YAC' and mention what was it used for. [3]

Answer 1

(a) The two major methodologies used in human genome project are:
(i) Expressed Sequence Tags: These are fragments of mRNA sequences derived through single sequencing reactions performed on randomly selected clones from cDNA libraries. [1]
(ii) Sequence Annotation: Sequencing the whole set of genome, coding or non-coding sequences and later assigning different regions with functions. [1]

(b) YAC stands for Yeast Artificial Chromosome [0.5]. It is used as a cloning vector. [0.5]