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Question

A segment of DNA, GCCAGGGGATG was translated into the oligopeptide arginine-serine- proline-tyrosine.
(a) What was the base sequence in the mRNA transcribed from the DNA segment?
(b) What are the codons for the 4 amino acids?
(c) If the first adenine in the DNA segment gets substituted by guanine, what will be the
1. mRNA transcript.
2. sequence of amino acids in the new oligopeptide.
3. anticodons on tRNA for the amino acids.

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Solution

a) The base sequence for the given sequence GCCAGGGGATG is CGGUCCCCUAC.
b) The codons for the 4 amino acids are
Arginine-CGT, CGC, CGA, CGG, AGA, AGG
Serine- TCT, TCC, TCA, TCG, AGT, AGC
Proline- CCT, CCC, CCA, CCG
Tyrosine- TAT, TAC
c)
1. The mRNA transcription would be-
CGGCCCCCUAC.
2. The sequence of amino acids will be-
arginine-glycine-proline-tyrosine
3. the anticodons will be
CGG-CCC-CC-TAC.

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