Question

A stained epidermal peel of a leaf appeared pinkish red under the microscope. The stain used was

• A. iodine.
• B. safranin.
• C. acetocarmine.
• D. none of these.

Answer 1

The correct option is B

safranin.

The correct answer is B.

Explanation of the correct option:

• Staining is a technique for raising contrast in material, usually on a tiny scale.
• Safranin turns the color of epidermal peel removed from a leaf into pinkish crimson color. The biological stain safranin is used in histology and cytology. In some staining techniques, safranin is used as a counterstain, turning all cell nuclei red.

Explanation of the incorrect options.

Option A:

• Potassium iodide reacts with starch to give a blue hue, which is used to identify a variety of polysaccharides like cellulose and glycogen.

Option C:

• The acetocarmine causes its regular staining effects, such as dark red nuclei and a less vivid red in the cytoplasm of some cells.

Option D:

• The safranin stain is used for staining epidermal leaf, causing it to appear. Hence, option B is correct.

Therefore, the epidermal leaf appeared pinkish red under a microscope after being stained with safranin.