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PCR in Diagnosis

A student is ...

Question

A student is performing a gel run for isolated DNA samples from various sources. These samples were digested by using a cocktail of restriction endonucleases; which region of gel will have greater DNA separation?

A

portion near to anode

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B

portion near to cathode

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C

The separation is uniform throughout the length

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D

It is size independent

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Solution

The correct option is A
portion near to anode

Gel electrophoresis is done for separating DNA fragments formed after restriction digestion. Since, a cocktail of restriction enzymes have been used in the given case, it will result in digestion of DNA segments at many places. Digestion at many places leads to the formation of numerous small fragments, so these fragments show greater movement under the influence of electric field towards the anode.

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