CameraIcon
CameraIcon
SearchIcon
MyQuestionIcon
MyQuestionIcon
1
You visited us 1 times! Enjoying our articles? Unlock Full Access!
Question

DNA is digested with a restriction endonuclease and subjected to gel electrophoresis.
Choose the correct statement w.r.t. to the above, among the following:

A
DNA fragments will migrate towards the cathode
No worries! We‘ve got your back. Try BYJU‘S free classes today!
B
Number of bands equals the number of restriction sites within the DNA
No worries! We‘ve got your back. Try BYJU‘S free classes today!
C
DNA fragments will migrate towards the anode
Right on! Give the BNAT exam to get a 100% scholarship for BYJUS courses
D
There is no correlation between the number of bands on the agarose gel and restriction sites in the DNA
No worries! We‘ve got your back. Try BYJU‘S free classes today!
Open in App
Solution

The correct option is C DNA fragments will migrate towards the anode
During electrophoresis, DNA migrates towards the positive terminal (anode) because DNA is a negatively charged molecule and its phosphate group is located at the backbone.
The number of fragments of DNA/number of bands on the gel formed are correlated to the number of restriction sites for the enzyme in the DNA by the formula "n+1", where "n" is the number of restriction sites.

flag
Suggest Corrections
thumbs-up
0
Join BYJU'S Learning Program
similar_icon
Related Videos
thumbnail
lock
Process Of Recombinant DNA Technology
BIOLOGY
Watch in App
Join BYJU'S Learning Program
CrossIcon