A transverse section of the stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem?
A
Orange and yellow
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B
Green and red
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C
Red and green
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D
Purple and orange
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Solution
The correct option is C Red and green Explanation
First green reacts with cytoplasm and cellulose content of the cell and stains them green.
Majority of the phloem tissue is made up of live parenchymal cells, hence it stains green.
Safranin reacts with lignin of sclerenchymatous tissue and stains them red.
Majority of the xylem tissue is dead sclerenchymatous tissue and hence, stains red.