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Question

A transverse section of the stem is stained first with safranin and then with fast green following the usual schedule of double staining for the preparation of a permanent slide. What would be the colour of the stained xylem and phloem?

A
Orange and yellow
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B
Green and red
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C
Red and green
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D
Purple and orange
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Solution

The correct option is C Red and green
Explanation
  • First green reacts with cytoplasm and cellulose content of the cell and stains them green.
  • Majority of the phloem tissue is made up of live parenchymal cells, hence it stains green.
  • Safranin reacts with lignin of sclerenchymatous tissue and stains them red.
  • Majority of the xylem tissue is dead sclerenchymatous tissue and hence, stains red.
Final answer: Option a. Red and Green

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