MyQuestionIcon

1

You visited us 1 times! Enjoying our articles? Unlock Full Access!

What is PCR

a Write the p...

Question

(a) Write the palindromic nucleotide sequence EcoRI recognises.

(b) Draw the vector DNA and a foreign DNA showing the sites where EcoRI has acted to form the sticky ends.

(c) Name the enzyme that helps in forming recombinant DNA.

Open in App

Solution

(c) DNA ligases join together foreign DNA and plasmid by forming phosphodiester bond.

Suggest Corrections

thumbs-up

0

Similar questions

Q. Eco RI is used to cut a segment of foreign DNA and that of a vector DNA to form a recombinant DNA. Show with the help of schematic diagrams.(i)
The set of palindromic nucleotide sequence of base pairs the Eco RI-will
recognise in both the DNA segments. mark the site at which Eco RI will act and-cut both the segments., (ii) Sticky ends formed on both the segments where the two DNA segments will join later to forma recombinant DNA.

Q. (a) Write the palindromic nucleotide for the following DNA segment:
5-G AA TTC-3
(b)Name the restriction endonuclease that recognises the sequence

(C)How are sticky-ends produced ? Mention their role.

Q. A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. Explain how the sticky ends are formed and get joined.

Q.
(a)EcoRI is a restriction endonuclease. How is it named so? Explain (b) Write the sequence of DNA bases that the enzyme recognises. Mention the point at which the enzyme makes a cut in the DNA segment.

Q. Each restriction endonuclease recognises a specific palindromic nucleotide sequence in the DNA. Explain.

View More

Join BYJU'S Learning Program

similar_icon

Related Videos

lock

Process Of Recombinant DNA Technology

BIOLOGY

Watch in App

explore_more_icon

Explore more

Standard XII Biology

Join BYJU'S Learning Program

CrossIcon