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Ligase

Among the fol...

Question

Among the following, select the tools of recombinant $D N A$ technology

a. Restriction enzymes
b. Polymerase enzymes
c. Ligases
d. Vectors
e. Host organisms

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Solution

The tools of recombinant $D N A$ technology include the following:

Restriction enzymes

Polymerase enzymes

Ligases

Vectors

Host organisms

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Similar questions

Q. Which of the following contains the key tools for recombinant DNA technology?
(i) Restriction endonucleases, ligases, vectors(ii) Ligases, host organism, polymerase enzymes(iii) Vectors, Taq polymerase, primers(iv) Restriction exonucleases, ligases, primers, bioreactors

Q. Recombinant DNA technology can be accomplished only if we have the following key tools i.e., reaction enzymes, polymerase enzyme, ligases and vectors.
State the functions of:
(a) Ligases
(b) Restriction enzymes

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Deoxyribonucleotides, buffer, E. coli, Taq DNA polymerase, primer, DNA probe, DNA cutting (restriction) enzymes, host cell

Q. Identify the correct sequence/steps involved in recombinant DNA technology:
A) Culturing the host cells in a medium at large scale
B) Isolation of DNA
C) Isolation of a desired DNA fragment
D) Transferring the recombinant DNA into the host
E) Ligation of the DNA fragment into a vector
F) Fragmentation of DNA by restriction endonucleases
G) Extraction of the desired product

Q. Study the following statements regarding recombinant DNA technology and select the incorrect ones

(i) Taq polymerase extends the primers using the nucleotides provided in the reaction

(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology

(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis

(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium

(v) To produce higher yields of the desired protein, host cells can be multiplied in a continuous culture

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