The correct option is D All of the above
Reduction
of inert atmospheric nitrogen to ammonia by living organisms is called
biological nitrogen-fixation. Only certain prokaryotic organisms (some bacteria
and cyanobacteria) are capable of fixing N2. Such microbes are called
N2-fixers. These N2-fixing microbes contain an exclusive enzyme,
Nitrogenase that is capable of reducing nitrogen. The nitrogen-fixing microbes
could be free-living or symbiotic in nature. Chlorobium, Rhodospirillum and Clostridium
are anaerobic, free-living, N2-fixing bacteria.