Question

An analysis of chromosomal DNA using southern hybridisation technique does not use __________.

• A. Blotting
• B. Autoradiography
• C. PCR
• D. Electrophoresis

Answer 1

Answer: (C)

PCR

• Analysis of DNA using southern hybridization uses electrophoresis or separation of DNA based on their charge and size.
• Then they are blotted on the nylon membrane, and autoradiography is done to see visible bands.
• PCR is done to amplify a specific segment of DNA. It is not part of southern hybridization.

So the correct answer is "PCR".