An analysis of chromosomal DNA using the Southern hybridization technique does not use:

Electrophoresis

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Blotting

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Autoradiography

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PCR

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Solution

The correct option is D PCR
An analysis of chromosomal DNA using the Southern hybridization technique involves the three following steps:

Gel electrophoresis

Blotting

Autoradiograph

PCR or polymerase chain reaction is used to amplify a single copy or a few copies DNA of interest. This process is not employed during a Southern hybridization technique.

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