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Question
Assertion :Gram negative bacteria do not retain the stain, when washed with alcohol. Reason: The outer membrane of Gram negative (-ve) bacteria contain lipopolysaccharides, a part of which is intergrated into membrane lipid.
Assertion :Gram negative bacteria do not retain the stain, when washed with alcohol. Reason: The outer membrane of Gram negative (-ve) bacteria contain lipopolysaccharides, a part of which is intergrated into membrane lipid.
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Solution
The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
When a decolourizer, such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A Gram-negative cell loses its outer lipopolysaccharide membrane and the inner peptidoglycan layer is left exposed. The crystal violet complexes are washed from the Gram-negative cell along with the outer membrane.
When a decolourizer, such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A Gram-negative cell loses its outer lipopolysaccharide membrane and the inner peptidoglycan layer is left exposed. The crystal violet complexes are washed from the Gram-negative cell along with the outer membrane.
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