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Question
Assertion :Use of chitinase enzyme, is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin.
Assertion :Use of chitinase enzyme, is necessary for isolation of DNA from yeast cells but not in case of Spirogyra. Reason: Fungal cell wall is made up of fungal cellulose or chitin.
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Solution
The correct option is A Both Assertion and Reason are correct and Reason is the correct explanation for Assertion
In order to use the DNA for genetic engineering processes, it must be in pure form, free from other macromolecules. This is termed DNA isolation and is the pioneer step. Since the DNA is enclosed within the membranes, it is required to break open the cell to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, lipids, etc. and from this mixture, DNA is purified. A release of DNA from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacteria), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall; plasma membrane degrading enzymes like lipase, etc., are also needed. Since yeast is a fungus and fungal cell wall is made of chitin (fungal cellulose), isolation of DNA necessarily requires the use of enzyme chitinase.
So, the correct answer is 'Both Assertion and Reason are correct and Reason is the correct explanation for Assertion'.
In order to use the DNA for genetic engineering processes, it must be in pure form, free from other macromolecules. This is termed DNA isolation and is the pioneer step. Since the DNA is enclosed within the membranes, it is required to break open the cell to release DNA along with other macromolecules such as RNA, proteins, polysaccharides, lipids, etc. and from this mixture, DNA is purified. A release of DNA from a cell is achieved by treating the cells or tissues with enzymes such as lysozyme (bacteria), cellulose (plant cells), chitinase (fungus), etc. These enzymes degrade cell wall; plasma membrane degrading enzymes like lipase, etc., are also needed. Since yeast is a fungus and fungal cell wall is made of chitin (fungal cellulose), isolation of DNA necessarily requires the use of enzyme chitinase.
So, the correct answer is 'Both Assertion and Reason are correct and Reason is the correct explanation for Assertion'.
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