Question

ATP produced during glycolysis is a result of substrate level phosphorylation. Explain.

Answer 1

Substrate level phosphorylation:

Substrate level phosphorylation is the process of formation of ATP by the transfer of phosphate groups from a substrate to ADP, rather from ADP and Pi (organic phosphate).

$\bullet$ Two substrate level phosphorylation takes place in glycolysis.

Step 7 of glycolysis:

Conversion of 1, 3 - bisphosphoglycerate to 3- phosphoglycerate.

$\bullet$ In this reaction, one phosphate group from the 1,3 -bisphosphoglycerate (substrate) is transferred to ADP for the formation of ATP.

Step 10 of glycolysis:

Conversion of phosphoenolpyruvate (PEP) to pyruvate.
In this reaction, the phosphate group from PEP (substrate) is transferred to ADP for ATP synthesis.