Question

Basis of DNA fingerprinting is

• A. DNA coiling
  
• B. Polymorphism in sequence/
  RFLP/satellite DNA
  
• C. Double helix
  
• D. Error in base sequence
  

Answer 1

The correct option is B Polymorphism in sequence/

RFLP/satellite DNA

DNA fingerprinting

DNA fingerprinting is a technique that allows the identification of variations which are unique to each organism using a VNTR probe that shows a very high degree of polymorphism.

Principle of DNA fingerprinting

DNA fingerprinting is based on the principle that allows the identification of variations which are unique to each organism using a VNTR probe that shows a very high degree of polymorphism.

Satellite DNA

Satellite DNA is a highly repetitive DNA sequence on chromosomes.

• These DNA sequences are unique to an organism.
• Thus, satellite DNA can be used as a probe in DNA fingerprinting.

RELP

RFLP stands for Restriction Fragment Length Polymorphism.

• It is a technique in which DNA is digested with a restriction enzyme.
• This produces multiple DNA fragments which are separated by agarose gel electrophoresis and further detected using a specific probe.
• RFLP is involved in DNA fingerprinting to identify specific DNA fragments of an individual.

Final answer: (c) Polymorphism in sequence/RFLP/satellite DNA