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Question
Can DNA fragments be seen directly?
Can DNA fragments be seen directly?
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Solution
DNA fragments:
- DNA is made up of nucleotides that help in the inheritance of genetic material from the parent to the daughter cells.
- DNA fragments can be obtained by cutting a DNA strand with a restriction enzyme.
- The restricted sample of DNA is subjected to electricity in a gel electrophoresis unit, stained with ethidium bromide, and viewed under UV light as bands.
- A single DNA fragment or even a small group of DNA fragments are not visible in the gel electrophoresis process.
- However, by comparing the bands in a sample to the DNA ladder, approximate sizes of DNA fragments can be determined.
DNA fragments:
- DNA is made up of nucleotides that help in the inheritance of genetic material from the parent to the daughter cells.
- DNA fragments can be obtained by cutting a DNA strand with a restriction enzyme.
- The restricted sample of DNA is subjected to electricity in a gel electrophoresis unit, stained with ethidium bromide, and viewed under UV light as bands.
- A single DNA fragment or even a small group of DNA fragments are not visible in the gel electrophoresis process.
- However, by comparing the bands in a sample to the DNA ladder, approximate sizes of DNA fragments can be determined.
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