Chilled ethanol is used in biotechnology for:

Creating transient pores in cell membrane

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DNA precipitation

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Cell culture preparation

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Protoplast fusion

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Solution

The correct option is B DNA precipitation
The purified DNA, after treatment with various enzymes, precipitates out after addition of chilled ethanol. This is viewed as a collection of fine threads in the suspension and is easily collected. The process is known as DNA spooling.

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