Question

Chromatography is based on the :

• A. Different rate of movement of the solvent in the column
• B. Different rate of movement of components in the mixture
• C. Separation of one solute from other constituents by being captured on the adsorbent
• D. None of the above

Answer 1

The correct option is B Different rate of movement of components in the mixture

Chromatography is based on the interaction between the solute molecules and active sites on the stationary phase. This attachment or interaction depends on the polarity of solutes. This technique proves the statement that “polar like polar”. Because if the stationary phase is more polar than the mobile phase then high polar compounds in the mixture will tightly bound to the stationary phase where as less polar compounds will lightly bound to the stationary phase. Less tightly bound compounds will be eluted out by the mobile phase earlier than the tightly bounded ones.Through this we can decide the solute in the solution.

Hence, the correct answer will be option (b).