Question

The separation in chromatography is mainly based on

• A. Differential partitioning between stationary and mobile phase
• B. pH of the stationary phase
• C. Temperature of the stationary phase
• D. All of the above

Answer 1

The correct option is A Differential partitioning between stationary and mobile phase

In partition chromatography, there is a solid matrix which is used to hold the stationary phase composed of a layer of the non-volatile substance. The mixture to be separated is carried by a gas or a liquid (mobile phase). The solutes distribute themselves between the mobile phase and the stationary phases, with the more soluble component in the mobile phase reaching the end of the chromatography column first.

Thus, the correct answer is option A.