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Qualities of a Good Vector

Cohen and Boy...

Question

Cohen and Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid having antibiotic resistance in the year

A

1963

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B

1967

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C

1972

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D

1982

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Solution

The correct option is C 1972
Stanley Cohen and Herbert Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance, in the year 1972.

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Q. Cohen and Boyer isolated an antibiotic resistance gene by cutting out a piece of DNA from a plasmid having antibiotic resistance in the year

Q. A piece of foreign DNA was inserted into a plasmid with an antibiotic resistance gene and lactose Z gene. The plasmid DNA was cut with a restriction enzyme, which splits the lactose Z gene and opens the circle. The foreign DNA was next inserted into the open restriction site of the plasmid. When the recombinant plasmid was introduced into bacterial cells and grown in the presence of antibiotic, some of the colonies turned blue in the presence of gene. The blue colonies contained

Q. Draw a schematic sketch of pBR

322

plasmid and label an antibiotic-resistant gene.

Q. The first recombinant DNA was constructed by linking an antibiotic-resistant gene with the native plasmid of

Q. Assertion (A): DNA ligase plays an essential role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.

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