Question

Commonly used vectors for human genome sequencing are ?

• A. T-DNA
• B. BAC and YAC
• C. expression vectors
• D. T/A cloning vectors

Answer 1

The correct option is B BAC and YAC

Commonly used vector for human genome sequencing are BAC (Bacterial Artificial Chromosome) and YAC. BAC is a DNA construct, based on a functional fertility plasmid (F plasmid) used for transforming and cloning in bacteria ( E.coli ). Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100–1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking. This is the process that was initially used for the Human Genome Project, however due to stability issues, YACs were abandoned for the use of Bacterial artificial chromosomes (BAC).

So the correct option is 'BAC and YAC'.