Question

Complete the steps for the separation and isolation of $DNA$ fragments.

(i) $DNA$ fragment separates in matrix of________.

(ii) $DNA$ fragments are stained with______

(iii) $DNA$ fragments are viewed under_________

(iv) Fragments are extruded from gel piece. This is called________

• A. Agarose gels,ethidium bromide, UV light, elution
• B. Polyacrylamide gels, ethidium bromide, UV light, elution
• C. Polyacrylamide gels, ethidium bromide, X-ray light, elution
• D. Agarose gels,ethidium bromide, X- ray light, extraction

Answer 1

The correct option is A Agarose gels,ethidium bromide, UV light, elution

A. DNA fragment separates in the matrix of agarose.

B. DNA fragments are stained with ethidium bromide.

C. DNA fragments are viewed under UV light.

D. DNA fragments are extruded from a gel piece. This is called elution.