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Question

_________ developed a technique for the separation of charged molecules under the influence of an electric field.

A
Tswett
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B
Tiselius
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C
Svedberg
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D
Sanger
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Solution

The correct option is B Tiselius

In 1931 Arne Tiselius developed a method called Electrophoresis, which employs electric current for separating a mixture of charged molecules. This separation of charged molecules totally depends on the size of the molecule and strength of the charge.

Mikhail Tswett in 1903 invented the procedure of chromatography while working on plant pigments. Tswett had successfully separated the mixture of plant pigments, including chlorophyll, by packing a column with finely ground calcium carbonate, using petroleum ether as the mobile phase.

Svedberg got the Nobel prize in 1926 for his work in the chemistry of colloids and also for his invention of ultracentrifuge. To separate the colloid from the mixture Svedberg used centrifugal force resulting in the settling of various cellular particles which depended on size and weight of the particle.

Sanger won his Nobel Prize for chemistry in 1958 for his work on the structure of protein. He was the first person to obtain a protein sequence. Sanger also proved that proteins were ordered molecules and by analogy, the genes and DNA that make these proteins.


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