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B
Tobacco plant
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C
Tomato plant
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D
None of the above
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Solution
The correct option is B Tobacco plant The method in which there is no involvement of the vector in the gene transfer is known as the direct gene transfer method. Different chemicals like PEG and calcium phosphate are used to make the cells competent which can take up the recombinant DNA. The method of direct gene transfer was first applied to the tobacco plant. An antibiotic resistance gene was transferred in the plant cell.