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Question

DNA fragments cut by restriction enzyme can be separated by


A

Agarose gel electrophoresis

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B
PCR
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C

Downstream processing

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D
ELISA
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Solution

The correct option is A

Agarose gel electrophoresis


PCR
Polymerase chain reaction (PCR) is a technique used for the in vitro amplification of the DNA fragments or gene of interest to create multiple copies.

ELISA
ELISA (Enzyme linked immunosorbent assay) is an immunological assay used to measure antibodies, antigens, proteins and glycoproteins in the blood.

Agarose gel electrophoresis
Agarose gel electrophoresis is the technique involving the separation of molecules based on their size and charge. For example, it is used for the separation of DNA fragments formed after treatment with endonucleases, based on their size and negative charge.

Downstream processing
After the biosynthetic phase, the recombinant products are separated and purified by the process called downstream processing. It involves the purification of the desired product, its packaging and marketing.

Final answer
(c) Agarose gel electrophoresis


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