Question

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

• A. Centrifugation
• B. Polymerase chain reaction
• C. Electrophoresis
• D. Restriction mapping

Answer 1

Answer: (C)

Electrophoresis

• DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by electrophoresis.
• The polymerase chain reaction is simply DNA replication in a test tube.
• Restriction mapping is the process of obtaining structural information on a piece of DNA by the use of restriction enzymes, e.g., endonucleases that recognize specific 4 to 8 base regions of DNA.

So, the correct answer is 'Electrophoresis'.