DNA primers are designed such that they specifically attach to the DNA fragments that we want to amplify.

True

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False

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Solution

The correct option is A True
DNA primers are 20-30 oligonucleotides long that are designed specifically to bind to the DNA fragments that we intend to amplify.

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Similar questions

Q. Pick the incorrect statements among the following.
(i) PCR is one of the techniques used in DNA fingerprinting.
(ii) PCR can be used for detection of a specific mutation or pathogen.
(iii) Alec Jeffreys won the Nobel Prize for the development of polymerase chain reaction (PCR).
(iv) DNA primers are designed such that they can attach anywhere on the DNA segment which has to be amplified.

Q. A DNA sequencing reaction was performed with the fragment

5 - X X X G C G A T C G Y Y Y Y - 3^{'}

as the template, dideoxy GTP, all the four

d N T P s

, and the required primers and enzyme.

X X X X

and

Y Y Y Y

in the given DNA fragment represent primer binding sites. The set of fragments obtained during the reaction will be (the primers are not shown in the amplified fragments).

Q. A biology student is performing PCR for the first time. She adds the right DNA fragments and its corresponding primers. She also adds DNA polymerase and free nucleotides. The reaction mixture is kept in a water bath that measures

37^{\circ} C

. But DNA amplification does not occur. Why?

Match the following enzymes of DNA replication with their respective functions:
$\begin{matrix} p . D N A p o l y m e r a s e & i . H e l p s u n z i p t h e D N A d o u b l e h e l i x q . D N A l i g a s e & i i . S e a l s n i c k s b e t w e e n O k a z a k i f r a g m e n t s r . D N A h e l i c a s e & i i i . L a y s R N A p r i m e r s t o w h i c h p o l y m e r a s e c a n a d d n u c l e o t i d e s s . P r i m a s e & i v . A d d s n u c l e o t i d e s t o t h e f r e e 3^{'} e n d o f R N A p r i m e r s \end{matrix}$

Q. A vector specifically designed for cloning

40 - 49

kb DNA fragment is?

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DNA primers are designed such that they specifically attach to the DNA fragments that we want to amplify.

The correct option is A TrueDNA primers are 20-30 oligonucleotides long that are designed specifically to bind to the DNA fragments that we intend to amplify.

The correct option is A True
DNA primers are 20-30 oligonucleotides long that are designed specifically to bind to the DNA fragments that we intend to amplify.