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Molecular Probes

DNA probes ar...

Question

DNA probes are used to detect the presence of nucleic acid sequences in DNA samples by hybridisation.

A

random

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B

same

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C

complementary

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Solution

The correct option is C complementary
Probes are complementary to the targetted gene of interest and also flouroscently labelled so that the gene of interest can be identified.

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Q. DNA probes are used to detect the presence of nucleic acid sequences in DNA samples by hybridisation.

Q. Assertion :Complementary pairing between nucleotides is used to diagnose presence of a specific DNA segment in a mixture. Reason: DNA probes having radioactive isotopes help to detect DNA by autoradiography.

Which of the following is/are true about Southern blotting?

Code:

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Q. Assertion :A genetic probe is helpful in the detection of specific DNA sequence. Reason: The genetic probe is a radiolabelled cDNA, which has a complementary base sequence, to DNA fragment which is to be detected.

Q. Arrange in a sequence the given protocol for DNA fingerprinting:
(a) Denaturating DNA

(c) Extraction of DNA

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(f) Hybridisation with the probe

(g) Exposure on film to make a DNA fingerprint

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