dNTPs differ in the structure from the normal NTPs and therefore stop the polymerase from moving forward in the replication fork and continue the replication process.

True

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False

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Solution

The correct option is A True
The reason dNTPs are used in the Sanger’s method is to terminate the replication process. Every time a dNTP is inserted into the DNA sequence the replication process gets terminated. This generates random sized DNA fragments. After several rounds of these fragments can be analyzed and used to determine the sequence of the DNA.

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Similar questions

Q. Which of the following enzyme is used to lay the foundation for DNA polymerase to start the addition of dNTPs during DNA replication?

During replication, DNA dependent RNA polymerase works in direction _{_________} and from Okazaki, the polarity of other parental strand is _{________}.

Q. How many of the following statements are correct ?

A. DNA dependent DNA polymerase requires DNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

B. DNA dependent DNA polymerase requires RNA primer for initiating replication but DNA dependent RNA polymerase does not require any primer for transcription.

C. During replication, unwinding of DNA double helix forms a fork-like structure called replication fork.

D. During transcription, unwinding of the DNA double helix by DNA dependent RNA polymerase forms a transcription bubble.

E. During transcription, unwinding of DNA double helix by DNA dependent RNA polymerase forms a transcription fork.

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Q. Which of the following statements is/are true with regards to DNA replication in E.coli?
a. It completes the process of replication within 18 minutes.
b. DNA polymerase catalyzes the polymerization very rapidly.
c. DNA polymerase catalyzes the reaction with a high degree of accuracy.

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