Question

Do restriction enzymes break hydrogen bonds?

Answer 1

Restriction enzymes:

1. A restriction enzyme is an isolated bacterial protein that cleaves DNA at certain sites in the sequence to produce DNA fragments with a known sequence at each end.
2. For several laboratory techniques, such as genetic engineering and recombinant DNA technologies, restriction enzymes are essential.
3. Although not necessarily in the same way, restriction enzymes cut through both nucleotide strands of the DNA to fragment it.
4. SmaI, a restriction enzyme that does this by cutting straight through DNA strands, creates DNA fragments with a flat or blunt end.
5. Restriction enzymes cleave the phosphodiester bond between the phosphate and the pentose sugar at a particular location in the sugar-phosphate backbone.