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Electroporation

Do the PCR te...

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Do the PCR technique is used for amplifying the gene of interest of the recombinant DNA or the gene of interest of only foreign DNA??

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Solution

foreign dna

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Q. Recombinant DNA, that contains our gene of interest is also called __ DNA.

Q. Arrange the given steps involved in recombinant DNA technology in the correct order.

A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme

Q. A gene of interest (GOI), amplified using PCR, can be isolated using agarose gel electrophoresis by ____i____ and the DNA is extracted out. This process is known as ____ii____.

Q. Match the following
Column I Column II
P. Gene cloning i. DNA from multiple sources
Q. Recombinant DNA ii.Copies of gene of interest
R. Genetic engineering iii. Vegetative propagation
S. Clones iv.Techniques used to modify the genome of an organism

Q. Match the following

\begin{matrix} C o l u m n I & C o l u m n I I P. Gene cloning & i. DNA from multiple sources. Q. Recombinant DNA & ii. Copies of gene of interest. R. Genetic engineering & iii. Vegetative propagation. S. Clones & iv. Techniques used to modify the genome of an organism. \end{matrix}

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