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Question

During DNA replication, Okazaki fragments are used to elongate :

A
The leading strand towards replication fork
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B
The lagging strand towards replication fork
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C
The leading strand away from replication fork
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D
The lagging strand away from replication fork
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Solution

The correct option is D The lagging strand away from replication fork
DNA replication is the process by which DNA makes copies of itself. During the process, the two strands of DNA separate and act as templates for the synthesis of new complementary strands. DNA polymerase is the enzyme that catalyses the polymerisation of the new DNA strands. They can catalyse the polymerisation only in 5' to 3' direction.
As a consequence, on one strand (the template with polarity 3' to 5'), the replication is continuous, while on the other (the template with polarity 5' to 3'), it is discontinuous. The discontinuously synthesised fragments are called Okazaki fragments. Okazaki fragments are formed on the lagging strand away from the replication fork and later joined by the enzyme DNA ligase.

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