Question

During staining, chromosomes get stained with

• A. Eosine
• B. Borax carmine
• C. Acetocarmine
• D. Safranin.

Answer 1

The correct option is C Acetocarmine

• Stains are used in the microscopic studies of different components and organelles within a cell.

A. Eosine is stain used to stain the cytoplasm of the cell because these acidic dyes bind to basic proteins in the cytoplasm of the cell.
B. Borax carmine consists of borax, carmine and water which produces a permanent red nuclear stain which stains the nucleus and the cytoplasm.

C. Acetocarmine is a saturated solution of carmine in 45% of acetic acid which is used for staining the unfixed chromosomes within the nucleus of a cell that is used to study different stages of the chromosomes in cell division.

D. Safranin is a basic counterstain dye used in histology and cytology.
So, the correct answer is 'Acetocarmine'.